PCR kits for mycoplasma detection

  • on average, 35% of cell cultures are infested by mycoplasma
  • mycoplasma contamination will substantially effect experimental results
  • mycoplasma contamination will interfere with cell-proliferation through both nutrient competition and toxic waste

                                 
VenorGeM® Mycoplasma Detection Kit

  • complies with highest standards for the rapid detection of mycoplasma contaminants
  • is a validated PCR test according to Eur. Ph. 2.6.21 with universal primers, to particularly detect the highly conservative 16s-rRNA region in mycoplasma
  • is specific for M. orale, M. hyorhinis, Acholeplasma laidlawii, M. arginini, M. Fermentans, and more than  20 additional mycoplasma species
  • is highly sensitive and reliable; detection limit is about 2-5 copies per 2 µl sample volume with a  detection efficiency of >95%.
  • meets a wide sample range including cell and virus cultures, and biopharmaceuticals
  • no complicated sample handling required; it only needs a few µl of cell culture supernatant
  • is suitable for the most variable number of samples
  • shows rapid yes/no results; handling time about 30 minutes; total test run 3 hours only
  • is based on standard lab equipment such as PCR cycler, polymerase and consumables
  • kit includes primer, nucleotides, buffers, and a positive control
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