PCR kits for mycoplasma detection
- on average, 35% of cell cultures are infested by mycoplasma
- mycoplasma contamination will substantially effect experimental results
- mycoplasma contamination will interfere with cell-proliferation through both nutrient competition and toxic waste
VenorGeM® Mycoplasma Detection Kit
- complies with highest standards for the rapid detection of mycoplasma contaminants
- is a validated PCR test according to Eur. Ph. 2.6.21 with universal primers, to particularly detect the highly conservative 16s-rRNA region in mycoplasma
- is specific for M. orale, M. hyorhinis, Acholeplasma laidlawii, M. arginini, M. Fermentans, and more than 20 additional mycoplasma species
- is highly sensitive and reliable; detection limit is about 2-5 copies per 2 µl sample volume with a detection efficiency of >95%.
- meets a wide sample range including cell and virus cultures, and biopharmaceuticals
- no complicated sample handling required; it only needs a few µl of cell culture supernatant
- is suitable for the most variable number of samples
- shows rapid yes/no results; handling time about 30 minutes; total test run 3 hours only
- is based on standard lab equipment such as PCR cycler, polymerase and consumables
- kit includes primer, nucleotides, buffers, and a positive control