Medium 199 - Liquid and Powdered

Medium 199, developed by Morgan, Morton and Parker, was one of the first chemically defined media used without a serum supplement for the continuous growth of primary chick embryo heart and fibroblast cells. With a serum supplement or in combination with less complex media, Medium 199 can support a broad spectrum of mammalian cells. Medium 199 with Earle's balanced salts is designed for a 5-10 % CO₂ atmosphere; Medium 199 with Hanks' balanced salts is designed for an air atmosphere in closed containers.