Trypsin - from porcine pancreas, Trypsin/EDTA solutions

: HeLa cells before and after trypsin treatment

Trypsin/EDTA solutions (0.05 or 0.02 % in PBS, without Ca²⁺, Mg²⁺) are used for passaging monolayer cultures. Trypsin affects the cell membrane and the cellular, and also the intercellular mucoproteins. Trypsin can be inhibited with the use of serum (see table "Comparison of Collagenase and Trypsin").

The Trypsin dry substance applied for the production of the different Trypsin solutions will be tested for mycoplasma and parvoviruses. Only in case both results are negative, the solutions are released.

to download: Comparison of Collagenase and Trypsin (table)

NEW (updated): Use of trypsin dry substance and trypsin/EDTA solutions

** Item needs dry ice when shipped.

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Trypsin - from porcine pancreas, Trypsin/EDTA solutions

Trypsin (1:250), 0.25 %

in PBS, without Ca²⁺, Mg²⁺

Trypsin (1:250), 2.5 %

in PBS, without Ca²⁺, Mg²⁺

Trypsin (1:250)/EDTA (0.25 %/0.02 %)

in PBS, without Ca²⁺, Mg²⁺

Trypsin/EDTA solution (0.05 %/0.02 %)

in PBS, without Ca²⁺, Mg²⁺

(10x) Trypsin (1:250)/EDTA solution (0.5 %/0.2 %)

in (10x) PBS, without Ca²⁺, Mg²⁺

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Trypsin - from porcine pancreas, Trypsin/EDTA solutions

Trypsin (1:250), 0.25 %

in PBS, without Ca2+, Mg2+

Trypsin (1:250), 2.5 %

in PBS, without Ca2+, Mg2+

Trypsin (1:250)/EDTA (0.25 %/0.02 %)

in PBS, without Ca2+, Mg2+

Trypsin/EDTA solution (0.05 %/0.02 %)

in PBS, without Ca2+, Mg2+

(10x) Trypsin (1:250)/EDTA solution (0.5 %/0.2 %)

in (10x) PBS, without Ca2+, Mg2+

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in PBS, without Ca²⁺, Mg²⁺

in PBS, without Ca²⁺, Mg²⁺

in PBS, without Ca²⁺, Mg²⁺

in PBS, without Ca²⁺, Mg²⁺

in (10x) PBS, without Ca²⁺, Mg²⁺