back to overview serum-free media

The serum-free preparation TNB 100 was optimized to cultivate neuro blastoma x glioma hybrid cells (108 CC 15). To prevent mutations of these cell hybrids, TNB 100 medium should contain HAT supplement in the usual concentration. Complete TNB 100 medium is formed by combining 100 ml basal medium and 2 ml of lipid-protein complex.

The medium does not contain any trypsin inhibitor. A trypsin inhibitor is hence required following trypsinsation of cultures. Culture conditions using TNB 100 medium should be: +37 °C, 10 % CO₂, at least 90 % relative humidity, and media exchange every 48 hours.

TNB 100 (w/o HAT) is well suited for normal neuronal cells (mouse, chick, and rat); however, the preparation may also be useful to study non-neuronal cells such as human cytotoxic T-cells.

Different from the original formulation, only 0.05 mg/l riboflavin are used due to risk of photo oxidative effects.