PFEK-1 Medium
With this serum and protein free medium, VERO cells proliferate without prior adaptation. VERO cells in protein free culture provide a sensitive substrate for the propagation of human pathogenic viruses. Cells were infected by several viruses (Coxsackie B4, Herpes simplex type 1 and 2, measles, and polio, type 1 to 3). Virus titres were comparable to those obtained in serum-supplemented media (see table).
Due to the very high calcium content of the medium, minor precipitates may occasionally be observed. However, this does not influence cell growth rates.