McCoy's 5 A modified liquid medium

McCoy's 5A medium was originally developed for the growth of Novikoff hepatoma cells. As modified by Iwakata and Grace, the increased levels of glucose, vitamins and peptone make McCoy's 5A a good general purpose medium for both primary, and established cell lines. Cells from biopsies (liver, intestine, skin, testes, bone marrow, spleen, lung and gingiva) have successfully been cultivated in this medium. To be used with 5 % CO₂ for optimum buffering.

Different from the original formulation, only 0.05 mg/l riboflavin are used due to risk of photo oxidative effects.