Serum is the fraction of blood remaining after coagulation and centrifugation of the cellular parts. It provides an extreme complex mixture of plasma proteins, growth factors, hormones etc. that makes it the commonly used supplement in cell culture. Components may vary according to origin, nutritive conditions and processing. Because of these natural variations it is necessary to do batch testing.
The high quality of Biochrom GmbH sera is based on the careful selection of raw serum, an optimized filtration process and sterile bottling: The aseptic filling of sera results in an aerea of the purity class A with a background environment of the purity class B according to the complementary GMP guidelines for the manufacture of sterile drugs, annex 1.

Growth support level of sera in cell and tissue culture is determined through extensive biological testing, including growth tests with a range of commonly used cell lines. All sera have to test negative for mycoplasma. The release of the sera is only effected after a negative result.

Fetal Bovine Serum (FBS) and Newborn Calf Serum are examined to exclude viruses such as Bovine Virus Diarrhoea (BVD-MD), Bovine Herpes Virus type 1 (BHV-1), and Parainfluenza Virus type 3 (PI-3). Test results have to prove negative with respect to cytopathogenicity and immunperoxidase staining. FBS is screened also for antibodies against these virus types.

For FBS, an independent laboratory raises a range of physicochemical data, provided to customers together with the sample.
Sample request FBS

Details of sera from Biochrom GmbH


Like all liquid products (media, additives), animal sera are sterilized using the membrane filtration technique with pore size <0.2 µm for sera in general, media and additives. For FBS, a filter pore size with 0.1 µm is used as standard.


Aliquots of each lot are inoculated into tryptone soya broth and thioglycollate. The full sterility control  procedure routinely takes 14 days. All products of animal source like serum or trypsin are screened through standard procedures for mycoplasma contamination.

Due to their sensitivity against enzymatic decomposition, animal sera have to be stored at temperatures -20°C. All data prove excellent stability, and performance of the sera for at least 60 months.


Samples are available for all sera, FBS in particular. Your reservation will be kept for the testing period. Shipment of sera is made at ≤ -20 °C on dry ice.

To prevent formation of high salt concentrations, the thawing procedure should be kept short. We recommend to warm up the bottle for about half an hour at room temperature (until a liquid film has formed), then put it into the water bath.
Detailed information of thawing and freezing of sera

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