Trypsin - from porcine pancreas, Trypsin/EDTA solutions
Trypsin/EDTA solutions (0.05 or 0.02 % in PBS, without Ca2+, Mg2+) are used for passaging monolayer cultures. Trypsin affects the cell membrane and the cellular, and also the intercellular mucoproteins. Trypsin can be inhibited with the use of serum (see table "Comparison of Collagenase and Trypsin").
The Trypsin dry substance applied for the production of the different Trypsin solutions will be tested for mycoplasma and parvoviruses. Only in case both results are negative, the solutions are released.