Trypsin - from porcine pancreas, Trypsin/EDTA solutions

HeLa cells before and after trypsin treatment

Trypsin/EDTA solutions (0.05 or 0.02 % in PBS, without Ca2+, Mg2+) are used for passaging monolayer cultures. Trypsin affects the cell membrane and the cellular, and also the intercellular mucoproteins. Trypsin can be inhibited with the use of serum (see table "Comparison of Collagenase and Trypsin"). 

The Trypsin dry substance applied for the production of the different Trypsin solutions will be tested for mycoplasma and parvoviruses. Only in case both results are negative, the solutions are released.

to download: Comparison of Collagenase and Trypsin (table)

Trypsin (1:250), 0.25 %

in PBS, without Ca2+, Mg2+

Product details +

Storage: 36 months at - 20 °C

Product Information


Production of this product has been discontinued. Clearance sale has already begun.     

Unit
Cat.No.
Unit price
L 2123
on request **

Trypsin (1:250), 2.5 %

in PBS, without Ca2+, Mg2+

Product details +

Storage: 36 months at - 20 °C

Product information


Production of this product has been discontinued. Clearance sale has already begun.

Unit
Cat.No.
Unit price
L 2133
on request **

Trypsin (1:250)/EDTA (0.25 %/0.02 %)

in PBS, without Ca2+, Mg2+

Product details +

Storage: 36 months at - 20 °C

Product information


A substitute is offered under the article number T4049 at Sigma-Aldrich https://www.sigmaaldrich.com/germany.html                     

Unit
Cat.No.
Unit price
L 2163
on request **

Trypsin/EDTA solution (0.05 %/0.02 %)

in PBS, without Ca2+, Mg2+

Product details +

Storage: 36 months at - 20 °C

Product Information


A substitute is offered under the article number T3924 at Sigma-Aldrich https://www.sigmaaldrich.com/germany.html     

Unit
Cat.No.
Unit price
L 2143
on request **

(10x) Trypsin (1:250)/EDTA solution (0.5 %/0.2 %)

in (10x) PBS, without Ca2+, Mg2+

Product details +

Storage: 36 months at - 20 °C

Product Information


A substitute is offered under the article number T4174 at Sigma-Aldrich https://www.sigmaaldrich.com/germany.html




 

Unit
Cat.No.
Unit price
L 2153
on request **

** Item needs dry ice when shipped.

Enter search key word:
Enter search key word: