Trypsin - from porcine pancreas

Trypsin/EDTA solutions (0.05 or 0.02 % in PBS, without Ca2+, Mg2+) are used for passaging monolayer cultures. Trypsin affects the cell membrane and the cellular, and also the intercellular mucoproteins. Trypsin can be inhibited with the use of serum. 

The Trypsin dry substance applied for the production of the different Trypsin solutions will be tested for mycoplasma and parvoviruses. Only in case both results are negative, the solutions are released.

NEW (updated): Use of trypsin dry substance and trypsin/EDTA solutions

Trypsin powder substance

Trypsin activity is min. 1250 USP units/mg. The activity of Trypsin dry substance is about five times more than the activity of Trypsin (1:250)*

Product details +

*In order to adapt the activity of this Trypsin dry substance to the activity of Trypsin 1:250, only 20 %, so 1/5, of the Trypsin dry substance with the activity of minimum 1250 USP units/mg is applied.

Storage: 24 months at +2 - +8 °C

Product Information


A substitute is offered under the article number T4799 at Sigma-Aldrich https://www.sigmaaldrich.com/germany.html



 

Unit
Cat.No.
Unit price
L 2103-20G
on request

** Item needs dry ice when shipped.

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