Papain: Non-animal alternative for trypsin (plant origin)
Papain is able to hydrolyse proteins and peptides preferably those with basic amino acids. The ready-to-use preparation (61.25 mg/l) in PBS w/o Ca2+, Mg2+ is derived from papaya fruit (Carica papaya).
If compared to the usual Trypsin, Papain preparation has a limited shelf life of 36 hrs at +2 - +8 °C. After thawing, Papain should consequently be divided into aliquots and re-frozen.