Collagenase, from Clostridium histolyticum (not sterile)
The treatment of tissue with collagenase effects a careful, selective reduction of the intercellular matrix and does not influence the growth ability of the cells. The raw collagenase offered by Biochrom GmbH is a mixture of different proteolytically efficient enzymes.
For optimal results, an exactly adjusted mixture of the proteolytic enzymes is necessary. For this purpose we offer 4 different types of Collagenase, CLS I, CLS II, CLS III and CLS IV. The last one is normally applied with other enzymes like trypsin, elastase, or hyaluronidase. The Trypsin or Trypsin/EDTA generally used in cell cultures attacks the matrix only slowly, causing moreover irreversible damages of the released cells.
to download: Comparison of Collagenase and Trypsin properties