Collagenase, from Clostridium histolyticum (not sterile)

The treatment of tissue with collagenase effects a careful, selective reduction of the intercellular matrix and does not influence the growth ability of the cells. The raw collagenase offered by Biochrom GmbH is a mixture of different proteolytically efficient enzymes.

For optimal results, an exactly adjusted mixture of the proteolytic enzymes is necessary. For this purpose we offer 4 different types of Collagenase, CLS I, CLS II, CLS III and CLS IV. The last one is normally applied with other enzymes like trypsin, elastase, or hyaluronidase. The Trypsin or Trypsin/EDTA generally used in cell cultures attacks the matrix only slowly, causing moreover irreversible damages of the released cells.

to download: Comparison of Collagenase and Trypsin properties

Collagenase type I, CLS I

Enzyme blends of collagenase, clostripain, with tryptic and proteolytic activities

Product details +

from Clostridium histolyticum. This preparation is recommended for liver, lung, adipose, and adrenal tissue. Specific activity is 125 to 250 Mandl units per ml of powder substance.

Storage: 24 months at + 2 - + 8 °C

Product Information


The production of this product will be transferred. Availability of the product under the same article number at Sigma-Aldrich from Q3/2019 on: https://www.sigmaaldrich.com/germany.html

Unit
Cat.No.
Unit price
C 1-28
on request

CLS type II, CLS II

Enzyme blending from collagenase, clostripain, with tryptic and proteolytic activities

Product details +

from Clostridium histolyticum. This preparation shows a high clostripain activity with the tryptic activity close to type I. Suitable for liver, bone, thyroid, heart, and salivary gland tissue. Specific activity is 125 to 250 Mandl units per milligram of powdered substance.

Storage: 24 months at + 2 - + 8 °C

Product Information


The production of this product will be transferred. Availability of the product under the same article number at Sigma-Aldrich from Q3/2019 on: https://www.sigmaaldrich.com/germany.html

Unit
Cat.No.
Unit price
C 2-28
on request

CLS type III, CLS III

Enzyme blending from collagenase, clostripain, with tryptic and proteolytic activities

Product details +

from Clostridium histolyticum. This preparation has a very low proteolytic and a normal collagenase activity. Suitable for mammary glandular tissue. Specific activity is 125 to 250 Mandl units per milligram of powdered substance.

Storage: 24 months at + 2 - + 8 °C

Product Information


Production of this product has been discontinued. Clearance sale has already begun.

Unit
Cat.No.
Unit price
C 3-28
on request

CLS type IV, CLS IV

Enzyme blending from collagenase, clostripain, with tryptic and proteolytic activities

Product details +

Recommended for the isolation of Islets of Langerhans. Specific activity is 125 to 250 Mandl units per milligram of powdered substance.

Storage: 24 months at + 2 - + 8 °C

Product Information


The production of this product will be transferred. Availability of the product under the same article number at Sigma-Aldrich from Q3/2019 on: https://www.sigmaaldrich.com/germany.html:

Unit
Cat.No.
Unit price
C 4-28
on request

** Item needs dry ice when shipped.

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