Biotase: Enzyme for separating cells

Compared to Trypsin, and to Papain (cat. no. L 2223), Biotase shows a far more maintaining enzyme effect on the sub-cultivation of adherent growing cells. The raw substance is extracted from invertebrates, having, therewith, compared to Trypsin from mammalian origin (bovine or porcine) a further benefit.

Working with Biotase the incubation time is longer in comparison with other enzyme preparations, but the important surface texture of the cells remains preserved (important for reattachment after passaging). The enzymatic effect is stopped by adding serum (FBS) or if necessary by dilution (washing).

Biotase

Product details +

Storage: 12 months at -20 °C

Product Information


A substitute is offered under the article number A6964 at Sigma-Aldrich https://www.sigmaaldrich.com/germany.html     

Unit
Cat.No.
Unit price
L 2193
on request **

** Item needs dry ice when shipped.

Enter search key word:
Enter search key word: