Biotase: Enzyme for separating cells
Compared to Trypsin, and to Papain (cat. no. L 2223), Biotase shows a far more maintaining enzyme effect on the sub-cultivation of adherent growing cells. The raw substance is extracted from invertebrates, having, therewith, compared to Trypsin from mammalian origin (bovine or porcine) a further benefit.
Working with Biotase the incubation time is longer in comparison with other enzyme preparations, but the important surface texture of the cells remains preserved (important for reattachment after passaging). The enzymatic effect is stopped by adding serum (FBS) or if necessary by dilution (washing).