RPMI 1640 liquid and powder medium

RPMI 1640 medium was developed by Moore and his co-workers while at Roswell Park Memorial Institute in 1966. Originally designed for the growth of human leukaemia cells in monolayer or suspension cultures using a serum supplement, it has since demonstrated universal use in the growth and support of a broad spectrum of mammalian and hybrid cells as well as in hybridoma fusion protocols.

For peripheral blood lymphocytes a mixture of 80 % RPMI 1640 and 20 % FBS is often used, and for murine myeloma cells a mixture of 85 % RPMI and 15 % FBS.

Different from the original formulation, only 0.05 mg/l riboflavin are used due to risk of photo oxidative effects.

RPMI 1640 liquid medium

with 2.0 g/l NaHCO3, without L-glutamine

Product details +

Storage: 18 months at +2 - +8 °C

Product Information


A substitute is offered under the article number R0883 at Sigma-Aldrich https://www.sigmaaldrich.com/germany.html

Unit
Cat.No.
Unit price
F 1215
on request

RPMI 1640 liquid medium with stable glutamine

with 2.0 g/l NaHCO3

Product details +

Storage: 18 months at +2 - +8 °C

Product Information


The production of this product will be transferred. Availability of the product under the same article number at Sigma-Aldrich from Q1/2020 on: https://www.sigmaaldrich.com/germany.html



 

Unit
Cat.No.
Unit price
FG 1215
on request

RPMI 1640 liquid medium with 20 mM HEPES

without NaHCO3, without L-glutamine

Product details +

Storage: 12 months at +2 - +8 °C

Product Information


A substitute is offered under the article number R5886 at Sigma-Aldrich https://www.sigmaaldrich.com/germany.html   

Unit
Cat.No.
Unit price
F 1235
on request

RPMI 1640 liquid medium with 20 mM HEPES, with stable glutamine

without NaHCO3

Product details +

Storage: 12 months at +2 - +8 °C
Product Information


Production of this product has been discontinued. Clearance sale has already begun.          

 




 

Unit
Cat.No.
Unit price
FG 1235
on request

RPMI 1640 liquid medium without phenol red

with 2.0 g/l NaHCO3, without L-glutamine

Product details +

Storage: 18 months at +2 - +8 °C

Product Information


A substitute is offered under the article number R7509 at Sigma-Aldrich https://www.sigmaaldrich.com/germany.html

Unit
Cat.No.
Unit price
F 1275
on request

RPMI 1640 liquid medium with stable gutamine

with 2.0 g/l NaHCO3, with 25 mM HEPES, with 5.5 g/l NaCl, with 5 mg/l phenol red

Product details +

Osmolality approx. 290 mOsm/kg water.
This RPMI modification is well suited for the cultivation of peripheral human lymphocytes.
Storage: 18 months at +2 - +8 °C

Product Information


The production of this product will be transferred. Availability of the product under the same article number at Sigma-Aldrich from Q1/2020 on: https://www.sigmaaldrich.com/germany.html

Unit
Cat.No.
Unit price
FG 1383
on request

RPMI 1640 luiquid medium with 5 mg/l phenol red

with 2.0 g/l NaHCO3, without L-glutamine

Product details +

Storage: 18 months at +2 - +8 °C

Product Information


A substitute is offered under the article number R0883 at Sigma-Aldrich https://www.sigmaaldrich.com/germany.html   

Unit
Cat.No.
Unit price
F 1295
on request

VLE-RPMI 1640 (Very Low Endotoxin) liquid medium

with 2.0 g/l NaHCO3, without L-glutamine

Product details +

Storage: 18 months at +2 - +8 °C
Product Information

A substitute is offered under the article number R0883 at Sigma-Aldrich https://www.sigmaaldrich.com/germany.html

Unit
Cat.No.
Unit price
F 1415
on request

VLE-RPMI 1640 (Very Low Endotoxin) liquid medium with stable glutamine

with 2.0 g/l NaHCO3

Product details +

Storage: 18 months at +2 - +8 °C

Product Information

A substitute is offered under the article number FG 1215 from Q1/2020 on at Sigma-Aldrich https://www.sigmaaldrich.com/germany.html   

Unit
Cat.No.
Unit price
FG 1415
on request

RPMI 1640 powder medium

without NaHCO3, with L-glutamine

Product details +

Storage: 24 months at +2 - +8 °C
Product Information


A substitute is offered under the article number R6504 at Sigma-Aldrich https://www.sigmaaldrich.com/germany.html   

Unit
Cat.No.
Unit price
T 121-01
on request

** Item needs dry ice when shipped.

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