Iscove's medium (incomplete)

Iscove's medium is a modification of DMEM using high glucose (4500 mg/l). It contains sodium pyruvate and additional vitamins, amino acids and selenium. Iscove's medium was originally designed for the serum-free growth of primary hemopoietic cells when supplemented with albumine, transferrin and soy bean lipids. When used in conjunction with serum, Iscove's supports growth of a broad spectrum of mammalian cells (lymphcytes, hybridomas and bone marrow). The medium containing a HEPES buffer and NaCHO3, is ideal for rapidly proliferating high density cell cultures in a 5 % CO2-atmoshphere.

Different from the original formulation, only 0.05 mg/l riboflavin are used due to risk of photo oxidative effects.

Complete Iscove’s medium is available upon request. This medium is also available as a standard in powder with a minimum order of 500 litres; pack sizes are 10 or 50 litres.

Iscove's liquid medium without L-glutamine

with 3.024 g/l NaHCO3, with 15 mg/l phenol red

Product details +

Storage: 12 months at +2 - +8 °C


A substitute is offered under the article number I3390 at Sigma-Aldrich https://www.sigmaaldrich.com/germany.html

Unit
Cat.No.
Unit price
F 0465
on request

Iscove's liquid medium with stable glutamine

with 3.024 g/l NaHCO3, with 15 mg /l phenol red

Product details +

Storage: 12 months at +2 - +8 °C


The production of this product will be transferred. Availability of the product under the same article number at Sigma-Aldrich from Q3/2019 on: https://www.sigmaaldrich.com/germany.html

Unit
Cat.No.
Unit price
FG 0465
on request

** Item needs dry ice when shipped.

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