Serum-free media for hybridoma culture ISF-1

ISF-1 is a serum-free, chemically defined optimized medium for hybridoma growth and monoclonal antibody production. With serum-free medium, cell culture is a defined process that provides greater consistency between experiments. Further advantages are batch to batch conformity, consistency in process, and freedom from concern regarding mycoplasma, and other contaminants. Using media without serum also makes it easier to purify a desired protein product from a culture after the cells secrete it into the supernatant, e.g. monoclonal antibodies produced in serum-free media will not be contaminated with unspecific bovine serum antibodies.

ISF-1 serum-free media supports growth and monoclonal antibody production of mouse hybridoma cell lines. ISF-1 does not contain exotic growth factors and is therefore cost efficient. In comparison to other commercially available serum-free media ISF-1 reveals superior performance with regard to cell growth, viability, and antibody productivity.

Custom packaging (bags 10-1000 litres) available upon request!

ISF-1 medium with stable glutamin

ready-to-use medium, serum-free

Product details +

Storage: 12 months at +2 - +8 °C

Instructions for use
Product Information

The production of this product will be transferred. Availability of the product under the same article number at Sigma-Aldrich from Q1/2020 on:


Unit price
F 9061-01
on request

** Item needs dry ice when shipped.

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