Ham's F-12 liquid medium

Developed by Ham, nutrient mixture Ham's F-12 has higher levels of amino acids, vitamins and trace elements. Putrescine and linoleic acid were added to the formulation. The medium was originally designed for the serum free growth of Chinese hamster ovary and lung cells. When used with whole or dialyzed serum or in combination with hormones and transferrin, Ham's F-12 is widely used to grow a broad spectrum of mammalian and hybridoma cells. Ham 's F-12 today is the medium of choice for clonal toxicity-assays using CHO cells (CHD-3 and CHL-1). For optimum buffering, use in a 5 % CO2 atmoshphere.

This medium is also available as a standard in powder with a minimum order of 500 litres; pack sizes are 10 or 50 litres.

Ham's F-12 liquid medium without l-glutamine

with 1.176 g/l NaHCO3, with 10 mg/l phenol red

Product details +

Storage: 18 months at +2 - +8 °C

Product Information


A substitute is offered under the article number N4888 at Sigma-Aldrich https://www.sigmaaldrich.com/germany.html

Unit
Cat.No.
Unit price
F 0815
on request

Ham's F-12 liquid medium with stable glutamine

with 1.176 g/l NaHCO3, with 10 mg/l phenol red

Product details +

Storage: 18 months at +2 - +8 °C

Product Information


The production of this product will be transferred. Availability of the product under the same article number at Sigma-Aldrich from Q1/2020 on: https://www.sigmaaldrich.com/germany.html           



Unit
Cat.No.
Unit price
FG 0815
on request

** Item needs dry ice when shipped.

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