Collagen G for optimum geling
Growth and differentiation of cells in vitro are significantly influenced by the substrate used (glass or plastic). If culture flasks are coated with attachment factors such as Fibronectin, Collagen, Gelatine or Polylysine, better cell growth is often achieved. Thus, for example, the survival time of hepatocytes on Collagen can be up to one month. Furthermore, due to attachment factors the tendency of many cells towards de-differentiation is prevented.
Biochrom GmbH sells two different collagens: Collagen A and Collagen G, acid-soluble calfskin collagens. Collagen G is used in the production of gels, to embed cells. Collagen G gels are suitable for use as a substrate for adherent cells in a cell culture vessel and as a floating matrix in or on cell culture medium. (Collagen A is used to coat culture flasks.)
Biochrom GmbH has updated the recommendations for the manufacture of gels with Collagen G with regard to the concentration and the necessary pH values of the starting solutions, to optimise geling. New features include tips for examining gels under a microscope and taking pictures.
Recommendation Collagen G