Collagen A: Coating culture flasks
Growth and differentiation of cells in vitro are significantly influenced by the substrate used (glass or plastic). If culture flasks are coated with attachment factors such as Fibronectin, Collagen, Gelatine or Polylysine, better cell growth is often achieved. Thus, for example, the survival time of hepatocytes on Collagen can be up to one month. Furthermore, due to attachment factors the tendency of many cells towards de-differentiation is prevented.
Biochrom GmbH sells two different collagens: Collagen A and Collagen G, acid-soluble calfskin collagens. Collagen A is used to coat culture flasks. (Collagen G is used in the production of gels, to embed cells.)
Biochrom GmbH has completely revised the recommendations for producing cell culture flask surfaces coated with Collagen A: Culture flasks can either be coated with monomeric Collagen A or with fibrillar Collagen A; monomeric Collagen A is invisible thanks to its smooth surface and is thus more suitable for photographing. In each case, what form the Collagen A is in depends on the particular pH, the ion concentration and the temperature of the solution. Biochrom GmbH recommends different procedures depending on whether you wish to use the culture flask immediately or whether you provide a longer period of storage.
Recommendation Collagen A