HEPES buffer

HEPES (hydroxyethyl-piperazineethane-sulfonic acid buffer) is an organic buffer and very stable. In the physiological area HEPES has an excellent buffer capacity in a pH range of 7.2 to 7.6. HEPES is apt for all cell culture systems with a high proliferation and cell density. On HEPES buffered cell culture media, the incubation in a CO2 incubator is not necessary.

 

How to use HEPES buffer:

  • • dissolve powder and adjust pH at 7.2 with sodium hydroxide
  • • add 10-20 ml of HEPES buffer per litre of medium (for very delicate cell cultures, use only 10 ml)
  • • a concentration of 20 mM HEPES requires 20 ml/l medium, for 25 mM use 25 ml/l
  • • if NaHCO3 is required, only add 10 %

Manufacturing scale GMP Process Chemicals with full regulatory support are also available within the complete Merck Millipore portfolio. For more information, please click here. 

1 M HEPES buffer (50x)

Product details +

Storage: 24 months at room temperature

Product Information


A substitute is offered under the article number H0887 at Sigma-Aldrich https://www.sigmaaldrich.com/germany.html


 

Unit
Cat.No.
Unit price
L 1613
on request

HEPES (powder)

MG 238.3

Product details +

Storage: 24 months at room temperature

Product Information


A substitute is offered under the article number H3784 at Sigma-Aldrich https://www.sigmaaldrich.com/germany.html


 

Unit
Cat.No.
Unit price
L 1603
on request

** Item needs dry ice when shipped.

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