G 418-BC: selective antibiotic for molecular research and gene transfer

G 418-BC is an antibiotic belonging to the aminoglycosides group. Due to its toxicity on bacteria, fungi, protozoa, and mammalian cells it is widely used as a selective agent in the molecular genetic research. Biochrom GmbH offers G 418-BC in powder form and as a sterile ready-to-use preparation.

mechanism: inhibits protein synthesis by disturbing the ribosome function

In order to use G 418, a derivative of neomycin, as selective antibiotic the neomycin resistance gene is required. This gene encodes an aminoglycoside 3‘-phosphotransferase (APT). APT is originally located on the bacterial transposon TN903. The heterologously expressed APT phosphorylates G 418 on the hydroxyls, which inhibits any interaction with ribosomes.

G 418-BC crystalline

not sterile (G 418 sulfate), activity > 500 U/mg

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to be dissolved in distilled water and sterile filtered
Storage: 24 months at +2 - +8 °C

Product Information


A substitute is offered under the article number G8168 at Sigma-Aldrich https://www.sigmaaldrich.com/germany.html  

Unit
Cat.No.
Unit price
A 291-25
on request

G 418-BC liquid (ready-to-use solution), sterile

activity 25000 U/ml (50 mg/ml), in distilled water

Product details +

dilute according to application
Storage: 24 months at +2 - +8 °C


Product Information

Instructions for use: antibiotic solutions are 100x concentrations and have been dissolved in WFI (quantity used: 1 ml per 100 ml medium).


A substitute is offered under the article number G8168 at Sigma-Aldrich https://www.sigmaaldrich.com/germany.html  

Unit
Cat.No.
Unit price
A 2912
on request

** Item needs dry ice when shipped.

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